Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
SIRT7 Protein, SIR2L7 Protein, Sirtuin type 7 Protein, SIR2-like protein 7 Protein, Sir2-related protein type 7 Protein, Sirtuin 7 Protein
NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor.
HDAC activity (HDAC Fluorimetric Assay -AK-500 from ELS). This assay is performed in two stages. In the first stage, the HDAC(s) acts on the Substrate (10 uM, 50 uM) (total volume of 50 ul). The second stage, is initiated by the addition of 50 ul of developer, stops HDAC activity and produces the fluorescent signal. The samples are read in a microplate reading fluorimeter, capable of excitation at a wavelength in the range 350~380nm and detection of emission light in the range 440~460nm.
Requested From: United States
Date Requested: 1/18/2017