Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.2 EU/µg protein (determined by LAL method).
SHH / Sonic Hedgehog
HEK 293 Cells
Greater than 90% by SDS-PAGE
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 5-40 µg/mL.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from sterile PBS, pH 7.4
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
SHH Protein, HHG-1 Protein, HLP3 Protein, Sonic hedgehog Protein, Sonic hedgehog homolog Protein, MCOPCB5 Protein, Sonic hedgehog protein Protein, HHG1 Protein, HPE3 Protein, SMMCI Protein, TPT Protein, TPTPS Protein
SHH / Sonic Hedgehog is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar.