Target
SELP / P-Selectin / CD62P
Synonyms
SELP | CD62P | CD62 | GMP-140 | GMRP | Granule membrane protein 140 | LECAM3 | P-selectin | PSEL | CD62P antigen | GMP140 | Granulocyte membrane protein | GRMP | PADGEM
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG Fc, C-terminus
Predicted Molecular Weight
The recombinant human SELP/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 971 amino acids and has a predicted molecular mass of 107 kDa. The apparent molecular mass of the reduced monomer is approximately 130-150 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Expression System
HEK 293 Cells
Purification
> 85 % as determined by SDS-PAGE
Bio-Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to SELP/Fc Chimera coated plates (10 µg/mL with 100 µL/well), > 80% cells will adhere after 1 hour at 37°C.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About SELP / P-Selectin / CD62P