Target
OPG / Osteoprotegerin
Synonyms
TNFRSF11B | OCIF | OPG | Osteoprotegerin | TR1
Conjugations
Unconjugated
Predicted Molecular Weight
20.0 kDa
AA Sequence
METFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGNSESTQK
Expression System
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
Determined by its ability to neutralize the stimulation of U937 cells treated with 10 ng/ml of soluble RANKL (sRANKL).
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Lyophilized from 10 mM Sodium Phosphate, pH 7.5
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris pH 7.5 to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About OPG / Osteoprotegerin