• Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Human NAMPT / Visfatin Protein (Recombinant His) (aa1-491) - LS-G3729

Catalog Size Price
LS-G3729-50 50 µg Unavailable

Most Popular NAMPT / Visfatin Proteins

Human NAMPT / Visfatin Protein (Recombinant) - LS-G118
E. coli Expression System
466 AA
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.1 ng/µg of protein (less than 1EU/µg).
Mouse NAMPT / Visfatin Protein (Recombinant His) - LS-G3596
E. coli Expression System
541 AA
Purified / Endotoxin Level: Less than 1.0 EU/µg protein (determined by LAL method).
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image
Human NAMPT / Visfatin Protein (Recombinant His) - LS-G3599
E. coli Expression System
511 AA
Purified / Endotoxin Level: Less than 1.0 EU/µg protein (determined by LAL method).
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image
Human NAMPT / Visfatin Protein (Recombinant His) (aa1-491) - LS-G3729
E. coli Expression System
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 1.0 EU/µg protein (determined by LAL method).
Functional Assay Image
Mouse NAMPT / Visfatin Protein (Recombinant His) (aa1-491) - LS-G3731
E. coli Expression System
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 1.0 EU/µg protein (determined by LAL method).
Functional Assay Image

100% Guaranteed
Recombinant Protein
NAMPT / Visfatin
~52kDa (SDS-PAGE)
Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
aa 1-491
E. coli
E. coli
Greater than 90% by SDS-PAGE
Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized, Salt free.
Reconstitute with distilled water.
Store lyophilized at -20°C for at least 1 year. Once reconstituted store at +4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid repeat freeze-thaw cycles.
For research use only.

About NAMPT / Visfatin

P43490 NM_005746 NP_005737.1

NAMPT Protein, PBEF Protein, Pre-B cell-enhancing factor Protein, VISFATIN Protein, VF Protein, NAmPRTase Protein, PBEF1 Protein

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma.

Functional Assay

Functional Assay
Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells. 10 ug/ml iNampt (human) (rec.) (His) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1 uM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.

Functional Assay

Functional Assay
Dimer formation of recombinant Nampt (Visfatin, PBEF) (AG-40A-0018). Purified Nampt (Visfatin, PBEF) was separated by SDS-PAGE and Western blot analysis was performed using rabbit anti-Nampt polyclonal antibody (AG-25A-0025). In the absence of DTT, Nampt (visfatin, PBEF) formed a homodimer, a homomultimer.

Functional Assay

Functional Assay
Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10 ul per 50 ul reaction mix were applied in the reaction mix (20 mmol/l Tris-HCl pH 7.4; 2,5 mmol/l ATP; 50 mmol/l NaCl; 12,5 mmol/l MgCl2; 2 mmol/l DTT; 0,5 mmol/l PRPP; 5 uMol/l 14C-nicotinamide) and incubated at 37°C for 1h. The 50 ul reaction mix was transferred into tubes containing 2ml of acetone and afterwards pipetted onto acetone-presoaked glass microfibre filters (GF/A 24 mm). After rinsing with 2 x 1ml acetone, filters were dried, transferred into vials with 6ml scintillation cocktail and radioactivity of 14C-NMN was quantified in a liquid scintillation counter. After subtraction of buffer values as background, cpm were normalized to 10^6 cells and the volume of enzyme preparation (10 ul). Mouse liver lysate at a concentration of 34.5 ug/ml was used as positive control in each assay. The positive control is mouse liver lysate at a concentration of 34,5 ug/ml - normally, which brings the most counts per minute (cpm) (contributed by Antje Garten and Dr. Kiess, University of Leipzig, Germany).

Requested From: 
Date Requested: 1/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number