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Human NAMPT / Visfatin Recombinant (His) Protein LS-G3729

Ordering

Wt. Vol. Conc. Price
50 µg - - $405
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NAMPT / Visfatin Proteins

Mouse NAMPT / Visfatin Recombinant Protein - LS-G4641
E. coli Expression System
None
Unpurified / Endotoxin Level: Less than 0.1 ng/µg of protein.
Rat NAMPT / Visfatin Recombinant Protein - LS-G4643
E. coli Expression System
None
Unpurified / Endotoxin Level: Less than 0.1 ng/µg of protein.
Human NAMPT / Visfatin Recombinant (His) Protein - LS-G16984
E. coli Expression System
His
Unpurified / Lyophilized

100% Guaranteed 100% Guaranteed
LS-G3729
Recombinant Protein
NAMPT / Visfatin
Human
~52kDa (SDS-PAGE)
Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
aa 1-491
E. coli
E. coli
His
Greater than 90% by SDS-PAGE
Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized, Salt free.
Reconstitute with distilled water.
Store lyophilized at -20°C for at least 1 year. Once reconstituted store at +4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid repeat freeze-thaw cycles.
For research use only.

About NAMPT / Visfatin

P43490 NM_005746 NP_005737.1

NAMPT Protein, PBEF Protein, Pre-B cell-enhancing factor Protein, VISFATIN Protein, VF Protein, NAmPRTase Protein, PBEF1 Protein

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma.

Functional Assay

Functional Assay
Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells. 10 ug/ml iNampt (human) (rec.) (His) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1 uM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.

Functional Assay

Functional Assay
Dimer formation of recombinant Nampt (Visfatin, PBEF) (AG-40A-0018). Purified Nampt (Visfatin, PBEF) was separated by SDS-PAGE and Western blot analysis was performed using rabbit anti-Nampt polyclonal antibody (AG-25A-0025). In the absence of DTT, Nampt (visfatin, PBEF) formed a homodimer, a homomultimer.

Functional Assay

Functional Assay
Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10 ul per 50 ul reaction mix were applied in the reaction mix (20 mmol/l Tris-HCl pH 7.4; 2,5 mmol/l ATP; 50 mmol/l NaCl; 12,5 mmol/l MgCl2; 2 mmol/l DTT; 0,5 mmol/l PRPP; 5 uMol/l 14C-nicotinamide) and incubated at 37°C for 1h. The 50 ul reaction mix was transferred into tubes containing 2ml of acetone and afterwards pipetted onto acetone-presoaked glass microfibre filters (GF/A 24 mm). After rinsing with 2 x 1ml acetone, filters were dried, transferred into vials with 6ml scintillation cocktail and radioactivity of 14C-NMN was quantified in a liquid scintillation counter. After subtraction of buffer values as background, cpm were normalized to 10^6 cells and the volume of enzyme preparation (10 ul). Mouse liver lysate at a concentration of 34.5 ug/ml was used as positive control in each assay. The positive control is mouse liver lysate at a concentration of 34,5 ug/ml - normally, which brings the most counts per minute (cpm) (contributed by Antje Garten and Dr. Kiess, University of Leipzig, Germany).
 

Requested From: United States
Date Requested: 12/9/2016

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