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Human MMP1 Protein (Recombinant) - LS-G137632

Catalog
Size
Price
LS-G137632-10
10 µg (0.2 mg/ml)
$285
LS-G137632-25
25 µg (0.2 mg/ml)
$405
LS-G137632-100
100 µg
$965
LS-G137632-500
500 µg
$2,910
Description
MMP1 Protein LS-G137632 is Recombinant Human MMP1 in Human It is biologically active and is low in endotoxin Less than 1 EU/µg (determined by LAL method)..
Type
Recombinant Protein
Target
MMP1
Species
Human
Predicted Molecular Weight
Predicted molecular mass of approximately 54 kD. The protein migrates at about 55 kD in DTT-reducing conditions and about 60 kD in non-reducing condition by SDS-PAGE.The N-terminal amino acid is Histidine.
Expression System
HEK 293 Cells
Source Species
Human
Purification
0
Bio-Activity
Human MMP-1 cleaves the peptide substrate Mca-KPLGL-Dpa-AR-NH2 with an activity above 200 pmol/min/µg.
Endotoxin
Less than 1 EU/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in 25 mM Tris, pH 7.5, 10 mM CaCl2, 150 mM NaCl
Storage
Store unopened at -20°C to -70°C for up to 6 months. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only.
About MMP1
P03956 NM_002421 NP_002412.1

Popular MMP1 Proteins

Protein
Source: E. coli Expression System
Tag: None
Format: Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.1 ng/µg of protein (less than 1EU/µg).

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Images

Western blot

Human MMP-1 (~54 kD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 1 h of activation, the mature form hMMP-1 (~45 kD) could be readily observed. Samples at reducing and non-reducing condition were resolved in a SDS-PAGE. Molecular weight markers at 250, 150, 100, 70, 55 kD were labeled here. Protein per lane: 2.5 µg
Human MMP-1 (~54 kD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 1 h of activation, the mature form hMMP-1 (~45 kD) could be readily observed. Samples at reducing and non-reducing condition were resolved in a SDS-PAGE. Molecular weight markers at 250, 150, 100, 70, 55 kD were labeled here. Protein per lane: 2.5 µg

Functional Assay

The activity of human MMP-1 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 3.125, 6.25, 12.5, 25, 50, 100 ng of activated human MMP-1.
The activity of human MMP-1 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 3.125, 6.25, 12.5, 25, 50, 100 ng of activated human MMP-1.

Western blot

Human MMP-1 (~54 kD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 1 h of activation, the mature form hMMP-1 (~45 kD) could be readily observed. Samples at reducing and non-reducing condition were resolved in a SDS-PAGE. Molecular weight markers at 250, 150, 100, 70, 55 kD were labeled here. Protein per lane: 2.5 µg
Human MMP-1 (~54 kD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 1 h of activation, the mature form hMMP-1 (~45 kD) could be readily observed. Samples at reducing and non-reducing condition were resolved in a SDS-PAGE. Molecular weight markers at 250, 150, 100, 70, 55 kD were labeled here. Protein per lane: 2.5 µg

Functional Assay

The activity of human MMP-1 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 3.125, 6.25, 12.5, 25, 50, 100 ng of activated human MMP-1.
The activity of human MMP-1 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 3.125, 6.25, 12.5, 25, 50, 100 ng of activated human MMP-1.

Western blot

Human MMP-1 (~54 kD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 1 h of activation, the mature form hMMP-1 (~45 kD) could be readily observed. Samples at reducing and non-reducing condition were resolved in a SDS-PAGE. Molecular weight markers at 250, 150, 100, 70, 55 kD were labeled here. Protein per lane: 2.5 µg
Human MMP-1 (~54 kD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 1 h of activation, the mature form hMMP-1 (~45 kD) could be readily observed. Samples at reducing and non-reducing condition were resolved in a SDS-PAGE. Molecular weight markers at 250, 150, 100, 70, 55 kD were labeled here. Protein per lane: 2.5 µg

Functional Assay

The activity of human MMP-1 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 3.125, 6.25, 12.5, 25, 50, 100 ng of activated human MMP-1.
The activity of human MMP-1 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 3.125, 6.25, 12.5, 25, 50, 100 ng of activated human MMP-1.

Western blot

Human MMP-1 (~54 kD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 1 h of activation, the mature form hMMP-1 (~45 kD) could be readily observed. Samples at reducing and non-reducing condition were resolved in a SDS-PAGE. Molecular weight markers at 250, 150, 100, 70, 55 kD were labeled here. Protein per lane: 2.5 µg
Human MMP-1 (~54 kD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 1 h of activation, the mature form hMMP-1 (~45 kD) could be readily observed. Samples at reducing and non-reducing condition were resolved in a SDS-PAGE. Molecular weight markers at 250, 150, 100, 70, 55 kD were labeled here. Protein per lane: 2.5 µg

Functional Assay

The activity of human MMP-1 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 3.125, 6.25, 12.5, 25, 50, 100 ng of activated human MMP-1.
The activity of human MMP-1 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 3.125, 6.25, 12.5, 25, 50, 100 ng of activated human MMP-1.

Requested From: United States
Date Requested: 9/15/2019