MMP1 Protein LS-G17319 is Recombinant Human MMP1 in Human with His tag(s). It is biologically active
MMP-1 Human Recombinant produced in HEK293 cells is a proform of the Human MMP1 (Met1-Asn469) and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-1 is purified by proprietary chromatographic techniques.
HEK 293 Cells
Greater than 95% by SDS-PAGE
The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is > 400 pMs/min/µg.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA). Activation Protocol: 1. Dilute MMP1 to 50 µg/ml in the Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5. 2. Activate MMP1 by adding APMA to a final concentration of 1 mM and 100 mM stock in DMSO. 3. Incubate at 37°C for 2 hours.
MES, NaCl, Brij35, Glycerol
Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
For research use only.