Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-G47818-100 | 100 µg | $480 |
| LS-G47818-1 | 1 mg | $2,324 |
1 of 3
2 of 3
3 of 3
Human KDR / VEGFR2 / FLK1 Protein (Recombinant His,C-terminus) (Met1-Glu764) - LS-G47818
Human KDR / VEGFR2 / FLK1 Protein (Recombinant His,C-terminus) (Met1-Glu764) - LS-G47818
Description:
KDR / VEGFR2 / FLK1 Protein LS-G47818 is a Recombinant Human KDR / VEGFR2 / FLK1 produced in HEK 293 Cells Met1-Glu764 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Description:
KDR / VEGFR2 / FLK1 Protein LS-G47818 is a Recombinant Human KDR / VEGFR2 / FLK1 produced in HEK 293 Cells Met1-Glu764 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
KDR / VEGFR2 / FLK1
Synonyms
KDR | CD309 | Fetal liver kinase 1 | Fetal liver kinase-1 | FLK-1 | FLK1 | Kinase insert domain receptor | Quek1 | VEGFR | VEGFR2 | Soluble VEGFR2 | VEGF receptor 2 | VEGFR-2 | CD309 antigen | VEGF-R2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Glu764
Predicted Molecular Weight
The secreted recombinant human VEGFR2 consists of 756 amino acids and predicts a molecular mass of 84.6 kDa. The apparent molecular mass of rhVEGFR2 is approximatly 120-130 in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 97 % as determined by SDS-PAGE
Bio-Activity
Using the Octet RED System,the affinity constant (Kd) of human VEGFR2-his bound to biotinylated human VEGF165 was 5.9nM.2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KDR / VEGFR2 / FLK1
Publications (0)
Customer Reviews (0)
Images
Binding
Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) bound to Anti-VEGFR2 Antibody was 1.9 µM.
Proliferation
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) bound to Anti-VEGFR2 Antibody was 1.9 µM.
Proliferation
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) bound to Anti-VEGFR2 Antibody was 1.9 µM.
Proliferation
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) bound to Anti-VEGFR2 Antibody was 1.9 µM.
Proliferation
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular KDR / VEGFR2 / FLK1 Proteins
Source:
Human
Tag:
Human IgG Fc, C-terminus
Source:
E. coli
Tag:
His-S
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
