Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
JAG1 Protein, AGS Protein, AHD Protein, Alagille syndrome Protein, CD339 antigen Protein, HJ1 Protein, Jagged1 Protein, JAGL1 Protein, Jagged Protein, Jagged 1 Protein, Protein jagged-1 Protein, AWS Protein, CD339 Protein, Soluble protein jagged Protein
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition. Macroscopic pictures.
Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH.