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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47273-100 | 100 µg | $403 |
| LS-G47273-1 | 1 mg | $1,697 |
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Human IL2RA / CD25 Protein (Recombinant His,C-terminus) (Met1-Cys213) - LS-G47273
Human IL2RA / CD25 Protein (Recombinant His,C-terminus) (Met1-Cys213) - LS-G47273
Description:
IL2RA / CD25 Protein LS-G47273 is a Recombinant Human IL2RA / CD25 produced in HEK 293 Cells Met1-Cys213 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Description:
IL2RA / CD25 Protein LS-G47273 is a Recombinant Human IL2RA / CD25 produced in HEK 293 Cells Met1-Cys213 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IL2RA / CD25
Synonyms
IL2RA | CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Cys213
Predicted Molecular Weight
The mature recombinant human IL2Ra/Fc is a disulfide-linked homodimeric protein generated by proteolytic removal of the signal peptide. The reduced monomer consists of 430 amino acids and has a predicted molecular mass of 48.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Ra/Fc monomer is approximately 72 kDa due to glycosylation.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Immobilized human IL2 at 10 µg/mL (100 µl/well) can bind human IL1RA. The EC50 of human IL1RA is 20-100 ng/mL
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL2RA / CD25
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Enzyme-Linked Immunosorbent Assay
Immobilized human IL2 at 10 µg/mL (100 µl/well) can bind human IL1RA. The EC50 of human IL1RA is 20-100 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Enzyme-Linked Immunosorbent Assay
Immobilized human IL2 at 10 µg/mL (100 µl/well) can bind human IL1RA. The EC50 of human IL1RA is 20-100 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Enzyme-Linked Immunosorbent Assay
Immobilized human IL2 at 10 µg/mL (100 µl/well) can bind human IL1RA. The EC50 of human IL1RA is 20-100 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Enzyme-Linked Immunosorbent Assay
Immobilized human IL2 at 10 µg/mL (100 µl/well) can bind human IL1RA. The EC50 of human IL1RA is 20-100 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
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Source:
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Tag:
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Source:
Human
Tag:
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
