Recombinant Protein

IL23

Human

Unmodified

The recombinant IL23 heterodimer of human IL23A/IL12B comprises 498 (181 + 317) amino acids and has a calculated molecular mass of 56.3 (20.1 + 36.2) kDa. The apparent molecular mass of rhIL23 heterodimer is approximately 22 & 45 kDa respectively in SDS-PAGE under reducing conditions.

HEK 293 Cells

Human

> 90 % as determined by SDS-PAGE

Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 µg/ml (100 µl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 µg/ml. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 µg/ml (100 µl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 µg/ml. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Reconstituted with sterile deionized water to 0.25 mg/mL.

Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles. Stable for 12 months.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.