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Human IL1RL1 Recombinant (Human IgG1 Fc) Protein (aa1-328) - LS-G3768


Wt. Vol. Conc. Price
50 µg - - Unavailable

Most Popular IL1RL1 Proteins

Human IL1RL1 Recombinant (Human IgG1 Fc) Protein (aa1-328) - LS-G3768
Human HEK 293 Cells
Human IgG1 Fc
Purified / Biologically Active / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Functional Assay Image
Human IL1RL1 Recombinant (FLAG) Protein (aa1-328) - LS-G3770
Human HEK 293 Cells
Purified / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Human IL1RL1 Recombinant (Human IgG1 Fc) Protein (aa19-328) - LS-G4028
Hamster-Chinese CHO Cells
Human IgG1 Fc
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.06 EU/µg protein (determined by LAL method).
Mouse IL1RL1 Recombinant (Mouse IgG2 Fc) Protein (aa27-342) - LS-G4113
Hamster-Chinese CHO Cells
Mouse IgG2 Fc
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.06 EU/µg protein (determined by LAL method).
Human IL1RL1 Recombinant (His) Protein (aa357-532) - LS-G15941
E. coli Expression System
Purified / Lyophilized

100% Guaranteed 100% Guaranteed
Recombinant Protein
~90kDa (SDS-PAGE)
Signal peptide and the sequence of isoform 2 of human ST2 (aa 1-328) are fused at the C-terminus to the Fc portion of human IgG1.
aa 1-328
HEK 293 Cells
Human IgG1 Fc
Greater than 90% by SDS-PAGE
Interacts with human IL-33.
Less than 0.1 EU/µg protein (determined by LAL method).
PBS, pH 7.2.
Store at +4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid repeat freeze-thaw cycles.
For research use only.

About IL1RL1

Q01638 NM_016232 NP_057316.3

IL1RL1 Protein, DER4 Protein, Growth stimulation-expressed Protein, IL33R Protein, Interleukin-1 receptor-like 1 Protein, Interleukin 1 receptor-like 1 Protein, ST2 Protein, ST2L Protein, ST2V Protein, FIT-1 Protein, Protein ST2 Protein, ST2 protein Protein

IL1RL1 is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Functional Assay

Functional Assay
hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Functional Assay

Functional Assay
Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.

Requested From: 
Date Requested: 4/25/2017

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