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Catalog Number Size Price
LS-G3768-50 50 µg $495 
IL1RL1 Protein - hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
IL1RL1 Protein - Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.
IL1RL1 Protein - hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
IL1RL1 Protein - Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.
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Human IL1RL1 Protein (Recombinant Human IgG1 Fc) (aa1-328) - LS-G3768

Human IL1RL1 Protein (Recombinant Human IgG1 Fc) (aa1-328) - LS-G3768

Description:
IL1RL1 Protein LS-G3768 is a Recombinant Human IL1RL1 produced in HEK 293 Cells aa 1-328 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method).
Price
Catalog Number
$495
LS-G3768-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
IL1RL1 Protein LS-G3768 is a Recombinant Human IL1RL1 produced in HEK 293 Cells aa 1-328 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method).

Specifications

Type
Recombinant Protein
Target
IL1RL1
Synonyms
IL1RL1 | DER4 | Growth stimulation-expressed | IL33R | Interleukin-1 receptor-like 1 | Interleukin 1 receptor-like 1 | ST2 | ST2L | ST2V | FIT-1 | Protein ST2 | ST2 protein | IL-1R4 | IL1R4
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Region
aa 1-328
Predicted Molecular Weight
~90kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Interacts with human IL-33.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
PBS, pH 7.2
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1RL1
Q01638 NM_016232 NP_057316.3

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Images

Functional Assay

IL1RL1 Protein - hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Functional Assay

IL1RL1 Protein - Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.
Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.

Functional Assay

IL1RL1 Protein - hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Functional Assay

IL1RL1 Protein - Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.
Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.

Functional Assay

IL1RL1 Protein - hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Functional Assay

IL1RL1 Protein - Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.
Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.

Functional Assay

IL1RL1 Protein - hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
hIL-33 binding to hST2-Fc confirmed by pull down assay. a) pull down of h-IL-33 by hST2-Fc. b) pull down of hST2-Fc by h-IL-33-His. 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Functional Assay

IL1RL1 Protein - Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.
Specific interaction of human ST2 with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/20/2021