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| Catalog Number | Size | Price |
|---|---|---|
| LS-G49854-100 | 100 µg | $480 |
| LS-G49854-1 | 1 mg | $2,333 |
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2 of 2
Human IL17A+IL17F Protein (Recombinant His,C-terminus) (Met1-Ala155) - LS-G49854
Human IL17A+IL17F Protein (Recombinant His,C-terminus) (Met1-Ala155) - LS-G49854
Description:
IL17A+IL17F Protein LS-G49854 is a Recombinant Human IL17A+IL17F produced in HEK 293 Cells Met1-Ala155 + Arg31-Gln163 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Description:
IL17A+IL17F Protein LS-G49854 is a Recombinant Human IL17A+IL17F produced in HEK 293 Cells Met1-Ala155 + Arg31-Gln163 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IL17A+IL17F
Synonyms
IL17AF | IL17A / IL17F Heterodimer
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Ala155 + Arg31-Gln163
Predicted Molecular Weight
The recombinant human IL17A & IL17F consists 280 amino acids and predicts a molecular mass of 31 kDa.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFa. The ED50 for this effect is 0.8-4 ng/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Functional Assay
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFa. The ED50 for this effect is 0.8-4 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Functional Assay
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFa. The ED50 for this effect is 0.8-4 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Functional Assay
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFa. The ED50 for this effect is 0.8-4 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Functional Assay
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFa. The ED50 for this effect is 0.8-4 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
