Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Proteins > IL-33 > LS-G3752
 
Print Page  
Catalog Number Size Price
LS-G3752-10 10 µg $302 
LS-G3752-50 50 µg $484 
IL-33 Protein - Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
IL-33 Protein - Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
IL-33 Protein - Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
IL-33 Protein - Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
1 of 2
2 of 2

Human IL-33 Protein (Recombinant His) (aa112-270) - LS-G3752

Human IL-33 Protein (Recombinant His) (aa112-270) - LS-G3752

Submit Review LSBio Guarantee
Description:
IL-33 Protein LS-G3752 is a Recombinant Human IL-33 produced in E. coli aa 112-270 with His tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$302
LS-G3752-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Publications Reviews Images Popular IL-33 Proteins Request SDS/MSDS

Overview

Description:
IL-33 Protein LS-G3752 is a Recombinant Human IL-33 produced in E. coli aa 112-270 with His tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
IL-33
Synonyms
IL33 | C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
His
Region
aa 112-270
Predicted Molecular Weight
~18kDa (SDS-PAGE)
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Activates human ST2-dependent NF-kappaB pathway.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
55 mM Tris-HCl, pH 8.2, 150 mM NaCl
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-33
O95760 NM_033439 NP_254274.1

Publications (0)

Submit a Publication for LS-G3752

Customer Reviews (0)

Submit a review for LS-G3752

Images

Functional Assay

IL-33 Protein - Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.

Functional Assay

IL-33 Protein - Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Functional Assay

IL-33 Protein - Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.

Functional Assay

IL-33 Protein - Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Functional Assay

IL-33 Protein - Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.

Functional Assay

IL-33 Protein - Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Functional Assay

IL-33 Protein - Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (human) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.

Functional Assay

IL-33 Protein - Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
Pull down assay: a) pull down of hIL-33-His by hST2-Fc b) pull down of hST2-Fc by hIL-33-His 5 ug of hST2-Fc (or control Fc protein), 2 ug of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4 degrees C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.

Popular IL-33 Proteins

IL-33 Protein - Recombinant Interleukin 33 By SDS-PAGE
Human IL-33 Protein (Recombinant His, N-Terminal) (aa1-270) - LS-G14580
Source: E. coli
Tag: His, N-Terminal
IL-33 Protein
Mouse IL-33 Protein (Recombinant) - LS-G131519
Source: Escherichia coli
Tag: None
IL-33 Protein - IL-5 induction by mouse IL-33 in splenocytes activated by anti-CD3 and anti-CD28. Data kindly provided Dr. Foo Y. Liew.
Mouse IL-33 Protein (Recombinant) (Ser109-Ile266) - LS-G137547
Source: E. coli
Tag: None
Product
Pig IL-33 Protein (Recombinant GST) (aa123-276) - LS-G20867
Source: E. coli
Tag: GST
Product
Human IL-33 Protein (Recombinant) - LS-G189
Source: E. coli
Tag: None
IL-33 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human IL-33 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G87081
Source: Human
Tag: Myc-DDK (Flag)

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com