Target
ICAM-1 / CD54
Synonyms
ICAM1 | BB2 | CD54 antigen | CD54 | ICAM-1 | p3.58
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant human ICAM1 consists of 459 amino acids and has a predicted molecular mass of 51 kDa. As a result of glycosylation, it migrates with an apparent molecular mass of 70-80 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to human ICAM1-coated plates (12.5 µg/ml, 100 µl/well), approximately 20-40% will adhere specifically.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile Tris 50mM, NaCl 100mM, pH 8.0, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee