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| Catalog Number | Size | Price |
|---|---|---|
| LS-G3964-50 | 50 µg | $379 |
Human FZD5 / Frizzled 5 Protein (Recombinant Human IgG1 Fc) - LS-G3964
Human FZD5 / Frizzled 5 Protein (Recombinant Human IgG1 Fc) - LS-G3964
Description:
FZD5 / Frizzled 5 Protein LS-G3964 is a Recombinant Human FZD5 / Frizzled 5 produced in HEK 293 Cells with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.01 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Description:
FZD5 / Frizzled 5 Protein LS-G3964 is a Recombinant Human FZD5 / Frizzled 5 produced in HEK 293 Cells with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.01 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
FZD5 / Frizzled 5
Synonyms
FZD5 | C2orf31 | DKFZP434E2135 | Frizzled family receptor 5 | Fz5 | Fzd-5 | FzE5 | Frizzled 5 | Frizzled-5 | Wnt receptor | Fz-5 | HFZ5
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Predicted Molecular Weight
~50kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Not Tested
Endotoxin
Less than 0.01 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with 50 µl sterile water.
Storage
Store lyophilized at -20°C for up to 6 months. Once reconstituted store at 4°C for immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
About FZD5 / Frizzled 5
Publications (0)
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Images
Functional Assay
Figure: Frizzled-5 (human):Fc (human) (rec.) (AG-40B-0133) efficiently inhibits Wnt3a activation. Methods: Inhibition of Wnt3a activation by Frizzled-5 (human):Fc (human) (rec.) was measured by using HEK293 cells transfected with a plasmid containing a luciferase gene under the control of a Wnt dependent promoter. Firefly luciferase activity was measured after incubation for 18h at 37 degrees C with 0.5ug/ml of Wnt3a with or without Frizzled-5 (h):Fc (concentrations indicated in the Figure). As negative control, medium only and 20ug/ml Frizzled-5 (h):Fc only did not show any signals (not shown). (Experiment performed by Alexey Koval, Prof. Katanaev Lab, University of Lausanne)
Functional Assay
Figure: Frizzled-5 (human):Fc (human) (rec.) (AG-40B-0133) efficiently inhibits Wnt3a activation. Methods: Inhibition of Wnt3a activation by Frizzled-5 (human):Fc (human) (rec.) was measured by using HEK293 cells transfected with a plasmid containing a luciferase gene under the control of a Wnt dependent promoter. Firefly luciferase activity was measured after incubation for 18h at 37 degrees C with 0.5ug/ml of Wnt3a with or without Frizzled-5 (h):Fc (concentrations indicated in the Figure). As negative control, medium only and 20ug/ml Frizzled-5 (h):Fc only did not show any signals (not shown). (Experiment performed by Alexey Koval, Prof. Katanaev Lab, University of Lausanne)
Functional Assay
Figure: Frizzled-5 (human):Fc (human) (rec.) (AG-40B-0133) efficiently inhibits Wnt3a activation. Methods: Inhibition of Wnt3a activation by Frizzled-5 (human):Fc (human) (rec.) was measured by using HEK293 cells transfected with a plasmid containing a luciferase gene under the control of a Wnt dependent promoter. Firefly luciferase activity was measured after incubation for 18h at 37 degrees C with 0.5ug/ml of Wnt3a with or without Frizzled-5 (h):Fc (concentrations indicated in the Figure). As negative control, medium only and 20ug/ml Frizzled-5 (h):Fc only did not show any signals (not shown). (Experiment performed by Alexey Koval, Prof. Katanaev Lab, University of Lausanne)
Functional Assay
Figure: Frizzled-5 (human):Fc (human) (rec.) (AG-40B-0133) efficiently inhibits Wnt3a activation. Methods: Inhibition of Wnt3a activation by Frizzled-5 (human):Fc (human) (rec.) was measured by using HEK293 cells transfected with a plasmid containing a luciferase gene under the control of a Wnt dependent promoter. Firefly luciferase activity was measured after incubation for 18h at 37 degrees C with 0.5ug/ml of Wnt3a with or without Frizzled-5 (h):Fc (concentrations indicated in the Figure). As negative control, medium only and 20ug/ml Frizzled-5 (h):Fc only did not show any signals (not shown). (Experiment performed by Alexey Koval, Prof. Katanaev Lab, University of Lausanne)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
