FGL1 / Hepassocin Protein LS-G116625 is Over-Expression Lysate Human FGL1 / Hepassocin in Human with Myc + DDK tag(s).
Over-Expression Lysate Protein
Predicted Molecular Weight
36.38 kDa Full Length
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FGL1 (NM_004467), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF, and 1mM Na3VO4).
HEK 293 Cells
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
Intended for use as a Western blot control. Spin down briefly and dilute with equal volume of 2xSDS Sample Buffer provided (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT). Prior to SDS-PAGE fractionation, boil the lysate for 5-10 minutes.
For research use only.
Available for shipment within the USA only.