FGF4 Protein LS-G55854 is Recombinant Human FGF4 in E. coli It is biologically active and is low in endotoxin Less than 1.0 EU/µg protein (determined by LAL method)..
Predicted Molecular Weight
Fibroblast growth factor 4 (FGF4) is a secreted growth factor that is predominantly expressed during bone morphogenesis and embryonic limb development. FGF4 is an important growth regulator for stem cells, fibroblasts, and endothelial cells. FGF4 contains a single N-linked glycosylation signal. However, in-vitro studies suggest that unglycosylated FGF4 is cleaved into 13 kDa and 15 kDa truncated proteins that have greater biological activity than the wild type 19 kDa FGF4 protein. Human FGF4 shares high homology and is cross-reactive with mouse FGF4. This product is produced with no animal or human origin raw products. All processing and handling employs animal free equipment and animal free protocols.
Greater than 95% by SDS-PAGE
Tested using: NR6R-3T3 Proliferation. ED50 less than or equal to 5 ng/mL (greater than or equal to2.0 x 10^5 units/mg).
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from sterile filtered 10 mM sodium phosphate, pH 7.5, 75 mM sodium chloride
Reconstitute in sterile water to 0.1 mg/ml
Store at -20°C to -80°C for up to 1 year. Store at 4°C for up to 1 month or up to 3 months at -20°C to -80°C after reconstitution.
For research use only.