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Catalog Number Size Price
LS-G3801-10 10 µg $330 
LS-G3801-50 50 µg $463 
FGF23 Protein - ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b

Human FGF23 Protein (Recombinant Human IgG1 Fc) (aa1-251) - LS-G3801

Human FGF23 Protein (Recombinant Human IgG1 Fc) (aa1-251) - LS-G3801

Description:
FGF23 Protein LS-G3801 is a Recombinant Human FGF23 produced in HEK 293 Cells aa 1-251 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$330
LS-G3801-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
FGF23 Protein LS-G3801 is a Recombinant Human FGF23 produced in HEK 293 Cells aa 1-251 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
FGF23
Synonyms
FGF23 | ADHR | Fibroblast growth factor 23 | FGF-23 | HYPF | Phosphatonin | PHPTC | HPDR2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Region
aa 1-251
Predicted Molecular Weight
60kDa-40kDa
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Activates ERK and FRS2alpha phosphorylation in Klotho expressing cells.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
PBS
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About FGF23
Q9GZV9 NM_020638 NP_065689.1

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Functional Assay

FGF23 Protein - ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b
ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b

Functional Assay

FGF23 Protein - ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b
ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b

Functional Assay

FGF23 Protein - ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b
ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b

Functional Assay

FGF23 Protein - ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b
ERK and FRS2alpha phosphorylation induced by FGF-21 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with hFGF-23-His, FGF-23-Fc and FGF-b (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Mock + hFGF23-Fc 1 ug/ml Lane 3: Mock + hFGF23-His 1 ug/ml Lane 4: Klotho + hFGF23-Fc 1 ug/ml Lane 5: Klotho + hFGF23-Fc 4 ug/ml Lane 6: Klotho + mCD137-Fc 1 ug/ml Lane 7: Klotho (non-treated) Lane 8: Klotho + 100ng/ml FGF-b

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/15/2024