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Human DLL4 Recombinant (Human IgG1 Fc) Protein (aa1-529) - LS-G3783


Wt. Vol. Conc. Price
10 µg - - $305
50 µg - - $395
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)

Most Popular DLL4 Proteins

Mouse DLL4 Recombinant (Human IgG1 Fc) Protein (aa1-532) - LS-G3829
Human HEK 293 Cells
Human IgG1 Fc
Unpurified / Lyophilized / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Functional Assay Image
Human DLL4 Recombinant Protein - LS-G4926
Human HEK 293 Cells
Unpurified / Endotoxin Level: Less than 0.1 ng/µg of protein (less than 1EU/µg).
Rat DLL4 Recombinant (His + GST) Protein (aa215-331) - LS-G15994
E. coli Expression System
His + GST
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image

100% Guaranteed 100% Guaranteed
Recombinant Protein
~80kDa (SDS-PAGE)
Signal peptide and extracellular domain of human DLL4 (aa 1-529) are fused at the C-terminus to the Fc portion of human IgG1.
aa 1-529
HEK 293 Cells
Human IgG1 Fc
Greater than 95% by SDS-PAGE
Inhibits adipogenesis of 3T3L-1 cells and mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.
Less than 0.01 EU/µg protein (determined by LAL method).
Lyophilized from PBS, 0.5% Trehalose
Reconstitute with 50 µl sterile water.
Store lyophilized at -20°C for at least 6 months. Once reconstituted store at +4°C for immediate use.
For research use only.

About DLL4

Q9NR61 NM_019074 NP_061947.1

DLL4 Protein, Delta-like 4 protein Protein, Delta like 4 Protein, Delta-like 4 (Drosophila) Protein, Delta-like 4 homolog Protein, Drosophila Delta homolog 4 Protein, Notch ligand DLL4 Protein, Delta 4 Protein, Delta ligand 4 Protein, Delta-like protein 4 Protein, Delta4 Protein, Hdelta2 Protein, Notch ligand delta-2 Protein

Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate.

Functional Assay

Functional Assay
Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.

Functional Assay

Functional Assay
Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.

Functional Assay

Functional Assay
Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.

Functional Assay

Functional Assay
Adipogenesis inhibition of 3T3L-1 cells.

Functional Assay

Functional Assay
Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Functional Assay

Functional Assay
50 ug of cell lysates derived from hDLL4-Fc or non-treated 3T3L1 cells, which had been either differentiated or undifferentiated, were subjected to western blot by using a mouse adiponectin antibody.

Requested From: United States
Date Requested: 2/26/2017

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