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Human DLL1 Protein (Recombinant) - LS-G128

Catalog Size Price
LS-G128-5 5 µg Unavailable
LS-G128-25 25 µg Unavailable

Most Popular DLL1 Proteins

Human DLL1 Protein (Recombinant) - LS-G128
Human HEK 293 Cells
522 AA
None
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.1 ng/µg of protein (less than 1EU/µg).
Human DLL1 Protein (Recombinant DDK) (aa1-545) - LS-G3781
Human HEK 293 Cells
DDK
Purified / Biologically Active / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were first incubated with a solution of anti-FLAG antibody (10 ug/ml) in PBS for 30 minutes at 37 degrees C. Coated plates were blocked with growth medium for at least 30 minutess, incubated with solution of DLL1 (human) (rec.) (5 ug/ml) or mCD137L-FLAG (5 ug/ml) in growth medium for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.
Human DLL1 Protein (Recombinant Human IgG1 Fc) (aa1-545) - LS-G3808
Hamster-Chinese CHO Cells
Human IgG1 Fc
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.01 EU/µg protein (determined by LAL method).
Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Mouse DLL1 Protein (Recombinant Human IgG1 Fc) (aa1-545) - LS-G3831
Human HEK 293 Cells
Human IgG1 Fc
Purified / Biologically Active / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Adipogenesis inhibition. Macroscopic pictures.
Human DLL1 Protein (Recombinant) - LS-G4920
Human HEK 293 Cells
232 AA
None
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.1 ng/µg of protein (less than 1EU/µg).

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LS-G128
Recombinant Protein
DLL1
Human
57.0-60.0 kDa
SGVFELKLQEFVNKKGLLGNRNCCRGGAGPPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGGGADSAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKVCNPGWKGPYCTEPICLPGCDEQHGFCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCKNGATCTNTGQGSYTCSCRPGYTGATCELGIDECDPSPCKNGGSCTDLENSYSCTCPPGFYGKICELSAMTCADGPCFNGGRCSDSPDGGYSCRCPVGYSGFNCEKKIDYCSSSPCSNGAKCVDLGDAYLCRCQAGFSGRHCDDNVDDCASSPCANGGTCRDGVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHERGHRYVCECARGYGGPNCQFLLPELPPGPAVVDLTEKLEGQGGPF
HEK 293 Cells
Human
None
Greater than 95% by SDS-PAGE and HPLC
Determined by the dose dependent growth suppression of the human acute monocytic leukemia cell line, THP-1. sDLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3.0-5.0 ug/ml.
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.5, 150 mM sodium chloride
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
For research use only.

About DLL1

O00548 NM_005618 NP_005609.3

DLL1 Protein, Delta-like 1 (Drosophila) Protein, Delta (Drosophila)-like 1 Protein, Drosophila Delta homolog 1 Protein, Delta-like protein 1 Protein, Delta1 Protein, H-Delta-1 Protein, DL1 Protein, Npa Protein

Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Requested From: 
Date Requested: 6/23/2018

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