Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition. Macroscopic pictures.
Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.