Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were first incubated with a solution of anti-FLAG antibody (10 ug/ml) in PBS for 30 minutes at 37 degrees C. Coated plates were blocked with growth medium for at least 30 minutess, incubated with solution of DLL1 (human) (rec.) (5 ug/ml) or mCD137L-FLAG (5 ug/ml) in growth medium for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.