Target
DDH / AKR1C1
Synonyms
AKR1C1 | 2-ALPHA-HSD | 20-alpha-HSD | Aldo-keto reductase C | Bile acid binding protein | Chlordecone reductase homolog | DDH1 | Dihydrodiol dehydrogenase 2 | DD1 | DDH | Dihydrodiol dehydrogenase 1/2 | DD1/DD2 | Dihydrodiol dehydrogenase 1 | H-37 | HAKRC | HBAB | Indanol dehydrogenase | MBAB
Conjugations
Unconjugated
AA Sequence
MGSSHHHHHHSSGLVPRGSHMDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEATKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCNSHRPELVRPALERSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCATWEAVEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNQRKLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAIDGLNRNVRYLTLDIFAGPPNYPFSDEY
Expression System
E. coli
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Specific activity: approximately 0.15-0.2 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein.
Presentation
20 mM Tris-HCl, pH 8.0, 1 mM DTT, 20% Glycerol
Storage
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee