Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
CX3CL1/fractalkine is a chemokine with a unique CX3C motif. Fractalkine is synthesized in endothelial cells as a membrane protein, and the N-terminal domain containing a CX3C motif is cleaved and secreted. Fractalkine is the first described cell-surface anchored chemokine and has potent mononuclear cell-directed adhesion and chemotactic properties. Fractalkine is involved in the pathogenesis of various chronic inflammatory diseases, such as rheumatoid arthritis and atherosclerosis.
Chemotaxis effect of human CX3CL1 (Fractalkine)-FLAG in THP-1 Cells. Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24 well transwell polystyrene membrane with 8 uM size pores. 100ng/ml, 500ng/ml, 1,000ng/ml, 2,500ng/ml and 5,000ng/ml of recombinant CX3CL1 were diluted in RPMI 1640 supplemented with 10mg/ml BSA, respectively. CX3CL1 samples were added to the lower chamber. THP-1 cells (0.5 X 10^6 cells per well), in the same media with CX3CL1, Soluble (human) (rec.) (Prod. No. AG-40A-0079), were added to the upper chamber. After 2 hour incubation at 37 degrees C in 5% CO2 humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from duplicate wells were enumerated on a hemacytometer by means light microscopy. Note: THP.1 cells are a very poor chemotactic responder with very low expression of human CX3CR1, which explains the high level of CX3CL1 needed for chemotaxis.
Requested From: United States
Date Requested: 2/20/2017