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Human CX3CL1 / Fractalkine Recombinant (FLAG) Protein (aa1-339) - LS-G3787


Wt. Vol. Conc. Price
10 µg - - Unavailable
50 µg - - Unavailable

Most Popular CX3CL1 / Fractalkine Proteins

Human CX3CL1 / Fractalkine Recombinant Protein - LS-G288
E. coli Expression System
76 AA
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.1 ng/µg of protein (less than 1EU/µg).
Rat CX3CL1 / Fractalkine Recombinant Protein - LS-G415
E. coli Expression System
76 AA
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.1 ng/µg of protein (less than 1EU/µg).
Human CX3CL1 / Fractalkine Recombinant (His) Protein - LS-G604
E. coli Expression System
97 AA
Purified / Endotoxin Level: Less than 1.0 EU/µg protein (determined by LAL method).
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image
Human CX3CL1 / Fractalkine Recombinant (FLAG) Protein (aa1-339) - LS-G3787
Human HEK 293 Cells
Purified / Biologically Active / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Functional Assay Image
Human CX3CL1 / Fractalkine Recombinant (Human IgG1 Fc) Protein (aa25-100) - LS-G4053
Hamster-Chinese CHO Cells
Human IgG1 Fc
Purified / Lyophilized / Endotoxin Level: Less than 0.06 EU/µg protein (determined by LAL method).

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Recombinant Protein
CX3CL1 / Fractalkine
~80kDa (SDS-PAGE)
Signal peptide and extracellular domain of human CX3CL1 (aa 1-339) are fused at the C-terminus to a FLAG®-tag.
aa 1-339
HEK 293 Cells
Greater than 90% by SDS-PAGE
Chemotaxis effect of soluble recombinant human CX3CL1 in THP-1 cells.
Less than 0.1 EU/µg protein (determined by LAL method).
Store at +4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid repeat freeze-thaw cycles.
For research use only.

About CX3CL1 / Fractalkine

P78423 NM_002996 NP_002987.1

CX3CL1 Protein, ABCD-3 Protein, C-X3-C motif chemokine 1 Protein, C3Xkine Protein, Chemokine ntt Protein, CXC3C Protein, FKN Protein, Fractalkine Protein, Neurotactin Protein, CXC3 Protein, NTN Protein, SCYD1 Protein, Small-inducible cytokine D1 Protein

CX3CL1/fractalkine is a chemokine with a unique CX3C motif. Fractalkine is synthesized in endothelial cells as a membrane protein, and the N-terminal domain containing a CX3C motif is cleaved and secreted. Fractalkine is the first described cell-surface anchored chemokine and has potent mononuclear cell-directed adhesion and chemotactic properties. Fractalkine is involved in the pathogenesis of various chronic inflammatory diseases, such as rheumatoid arthritis and atherosclerosis.

Functional Assay

Functional Assay
Chemotaxis effect of human CX3CL1 (Fractalkine)-FLAG in THP-1 Cells. Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24 well transwell polystyrene membrane with 8 uM size pores. 100ng/ml, 500ng/ml, 1,000ng/ml, 2,500ng/ml and 5,000ng/ml of recombinant CX3CL1 were diluted in RPMI 1640 supplemented with 10mg/ml BSA, respectively. CX3CL1 samples were added to the lower chamber. THP-1 cells (0.5 X 10^6 cells per well), in the same media with CX3CL1, Soluble (human) (rec.) (Prod. No. AG-40A-0079), were added to the upper chamber. After 2 hour incubation at 37 degrees C in 5% CO2 humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from duplicate wells were enumerated on a hemacytometer by means light microscopy. Note: THP.1 cells are a very poor chemotactic responder with very low expression of human CX3CR1, which explains the high level of CX3CL1 needed for chemotaxis.

Requested From: 
Date Requested: 4/25/2017

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