Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
367 kDa (Pro) and 29 kDa (Mature). DTT-reduced protein migrates as 43 kDa and 34 kDa due to glycosylation
HEK 293 Cells
Greater than 95% by SDS-PAGE
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100 µl reaction mixture containing 25 mM MES, pH 5.0, 0.01 µg rhCathepsin B, and 10 µM reaction substrate. The specific activity is >2800 pmol/min/µg.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5-8% mannitol or trehalose
Centrifuge the vial before opening. Reconstitute in sterile PBS, pH 7.4 to 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, store at -20°C.
CTSB Protein, Cathepsin B1 Protein, Cathepsin B Protein, Cathepsins B Protein, CPSB Protein, Cysteine protease Protein, Preprocathepsin B Protein, APP secretase Protein, APPS Protein
Cathepsin B is a lysosomal cysteine proteinase synthesized as a zymogen of 39-47 kilodaltons (kD), which is subsequently converted into an active single- chain form of 33 kD (CB33) and, by additional processing, into the active 2-chain form containing a heavy chain of 27-29 kD (CB(27-29)) and a light chain of 4-6 kD.