Recombinant Protein

CSF1R / CD115 / FMS

Human

Unmodified

The recombinant human M-CSFR contains 504 amino acids after the removal of signal peptide with a calculated molecular mass of 56 kDa. As a result of glycosylation, it migrates as an approximately 85-95 kDa protein in SDS-PAGE under reducing conditions.

HEK 293 Cells

Human

> 98 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human CSF1R at 10 µg/mL (100 µl/well) can bind biotinylated human CSF-1, The EC50 of biotinylated human CSF-1 is 8 ng/mL. Measured by its ability to inhibit the human CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 6-30 µg/mL in the presence of 3 ng/ml Recombinant Human M-CSF.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to included instructions for detailed information.

Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P07333 NCBI: NM_005211 NP_005202.2