Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself.
After denaturation of recombinant secretory clusterin in the presence of beta -mercaptoethanol followed by boiling, two distinct bands, 60kDa and 35kDa, were separated on SDS-PAGE. The two bands were extracted and subjected to determination of their NH2-terminal sequences. The larger band represents the uncleaved (unprocessed) secretory clusterin whose NH2-terminal amino acids are DQTVS corresponding to a predicted NH2-terminus. The smaller band is a mixture of the NH2-terminal sequences, SLMPF and DQTVS, which correspond to the NH2-terminal sequences of the alpha and beta chain of the processed secretory clusterin, respectively.