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Proteins > CD40L > LS-G3877
 
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Catalog Number Size Price
LS-G3877-10 10 µg $435 
CD40L Protein - CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.

Human CD40L Protein (Recombinant DDK (Flag)-Human ACRP30headless aa 18-111) (aa116-261) - LS-G3877

Human CD40L Protein (Recombinant DDK (Flag)-Human ACRP30headless aa 18-111) (aa116-261) - LS-G3877

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Description:
CD40L Protein LS-G3877 is a Recombinant Human CD40L produced in CHO Cells aa 116-261 with DDK (Flag)-Human ACRP30headless aa 18-111 tag(s). It is biologically active and is low in endotoxin; Less than 0.01 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$435
LS-G3877-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Publications Reviews Images Popular CD40L Proteins Request SDS/MSDS

Overview

Description:
CD40L Protein LS-G3877 is a Recombinant Human CD40L produced in CHO Cells aa 116-261 with DDK (Flag)-Human ACRP30headless aa 18-111 tag(s). It is biologically active and is low in endotoxin; Less than 0.01 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
CD40L
Synonyms
CD40LG | CD154 | CD154 antigen | CD40-L | CD40 ligand | CD40L | gp39 | HIGM1 | IGM | IMD3 | T-BAM | TNFSF5 | CD40 antigen ligand | CD54 | HCD40L | T-B cell-activating molecule | T-cell antigen Gp39 | TNF-related activation protein
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
DDK (Flag)-Human ACRP30headless aa 18-111
Region
aa 116-261
Predicted Molecular Weight
~35-40kDa (SDS-PAGE)
Expression System
CHO Cells
Source Species
Hamster-Chinese
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).
Endotoxin
Less than 0.01 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with 100 µl sterile water.
Storage
Store lyophilized at -20°C for up to 6 months. Once reconstituted store at 4°C for immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD40L
P29965 NM_000074 NP_000065.1

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Images

Functional Assay

CD40L Protein - CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.
CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.

Functional Assay

CD40L Protein - CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.
CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.

Functional Assay

CD40L Protein - CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.
CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.

Functional Assay

CD40L Protein - CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.
CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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