Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
TNFSF5 is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in inability of immunoglobulin class switch and is associated with hyper IgM syndrome.
CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.
Requested From: United States
Date Requested: 10/27/2016