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Human CD40L Recombinant (FLAG + human ACRP30headless aa 18-111) Protein (aa116-261) - LS-G3877


Wt. Vol. Conc. Price
10 µg - - Unavailable

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Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image

100% Guaranteed 100% Guaranteed
Recombinant Protein
~35-40kDa (SDS-PAGE)
Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
aa 116-261
CHO Cells
FLAG + human ACRP30headless aa 18-111
Greater than 95% by SDS-PAGE
Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).
Less than 0.01 EU/µg protein (determined by LAL method).
Lyophilized from PBS.
Reconstitute with 100 µl sterile water.
Store lyophilized at -20°C for at least 6 months. Once reconstituted store at +4°C for immediate use.
For research use only.

About CD40L

P29965 NM_000074 NP_000065.1

CD40LG Protein, CD154 Protein, CD154 antigen Protein, CD40-L Protein, CD40 ligand Protein, CD40L Protein, gp39 Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, CD40 antigen ligand Protein, CD54 Protein, HCD40L Protein, T-B cell-activating molecule Protein, T-cell antigen Gp39 Protein, TNF-related activation protein Protein

TNFSF5 is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in inability of immunoglobulin class switch and is associated with hyper IgM syndrome.

Functional Assay

Functional Assay
CD40L (human) (multimeric) (rec.) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100 ul RPMI supplemented with 10% FCS) for 24 hours at 37 degrees C with the indicated concentration of CD40L (human) (multimeric) (rec.) or CD40L (h) in the presence and absence of 1 ug/ml Enhancer (Prod. No AG-35B-0001). Cells were washed with PBS and stained with 2 ul each CD86-PE and CD19-FITC in 50 ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 minutes. at 4 degrees C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.

Requested From: 
Date Requested: 3/28/2017

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