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Human CCL20 / MIP-3-Alpha Protein (Recombinant) - LS-G285
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.1 ng/µg of protein.
LS-G285
Recombinant Protein
CCL20 / MIP-3-Alpha
Human
8.0 kDa
ASNFDCCLGY
TDRILHPKFI
VGFTRQLANE
GCDINAIIFH
TKKKLSVCAN
PKQTWVKYIV
RLLSKKVKNM
E. coli
E. coli
None
Greater than 98% by SDS-PAGE and HPLC
Determined by its ability to chemoattract human T cells using a concentration of 10.0-50.0 ng/ml.
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Sterile filtered, lyophilized with no additives.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid repeat freeze-thaw cycles.
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
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