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Human CALR / Calreticulin Recombinant (His) Protein - LS-G3822


Wt. Vol. Conc. Price
10 µg - - Unavailable
50 µg - - Unavailable

Most Popular CALR / Calreticulin Proteins

Human CALR / Calreticulin Recombinant (His) Protein - LS-G773
E. coli Expression System
Unpurified / Lyophilized
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image
Human CALR / Calreticulin Recombinant (His) Protein - LS-G5330
E. coli Expression System
Unpurified / Lyophilized / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Human CALR / Calreticulin Recombinant (His) Protein - LS-G20210
E. coli Expression System
Unpurified / Lyophilized

100% Guaranteed 100% Guaranteed
Recombinant Protein
CALR / Calreticulin
~55kDa (SDS-PAGE)
Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag.
E. coli
E. coli
Greater than 90% by SDS-PAGE
Less than 1.0 EU/µg protein (determined by LAL method).
55 mM Tris-HCl, pH 8.2, 150 mM NaCl
Store at +4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid repeat freeze-thaw cycles.
For research use only.

About CALR / Calreticulin

P27797 NM_004343 NP_004334.1

CALR Protein, Calregulin Protein, CC1qR Protein, CRP55 Protein, CRT Protein, CRTC Protein, ERp60 Protein, Grp60 Protein, RO Protein, SSA Protein, Calreticulin Protein, HACBP Protein

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors.

Functional Assay

Functional Assay
Flow cytometric analysis of CRT on the cell surface 3.10^5 EL4 Thymoma cells, growing in suspension in RPMI 1640 (Gibco) supplemented medium were plated in 12-well plates and treated with mitomycin C (30mM, Sanofi Aventis) or cisplatin (25mM, Sigma) for 4h. Cells were harvested, washed once with cold PBS and possibly resuspended in 200mL of cold PBS containing 1mg of recombinant Calreticulin for 30 minutesutes on ice. After one wash with cold PBS, cells were fixed in 0.25% paraformaldehyde (PFA) in PBS for 5 minutesutes. After washing again once with cold PBS, cells were incubated for 30 minutes with primary antibody, diluted in cold blocking buffer (2% FBS in PBS), followed by washing and incubation with the Alexa488-conjugated monoclonal secondary antibody in blocking buffer (30 minutes). Each sample was then analyzed by FACScan (Becton Dickinson) to identify cell-surface Calreticulin. Secondary antibody alone was used as an isotype control, and the fluorescent intensity of stained cells was gated on propridium iodide (PI) negative cells.Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Functional Assay

Functional Assay
Immunofluorescence Cells were possibly incubated with rCRT and mitoxanthron (1mM, Sigma) treated cells were used as positive control. Pictures courtesy of Prof. Guido Kroemer, INSERM, Paris.

Requested From: 
Date Requested: 3/23/2017

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