Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
41.2 kDa. DTT-reduced protein migrates as 53-54 kDa due to glycosylation
HEK 293 Cells
Greater than 95% by SDS-PAGE
Measured by its esterase activity. The specific activity is measured with 1 mM 4-Nitrophenyl acetate and 2.5 µg enzyme at 400 nm in 100 µL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is > 15 pmoles / min / µg.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from 20 mM MES, 100 mM NaCl, pH 6.5, mannitol or trehalose
Centrifuge the vial before opening. Reconstitute in sterile PBS, pH 7.4 to 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, store at -20°C.
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization.