Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 1.0 EU/µg protein (determined by LAL method).
CA10 / Carbonic Andhydrase X
32.6 kDa. DTT-reduced protein migrates as 37 kDa due to glycosylation
HEK 293 Cells
Greater than 95% by SDS-PAGE
Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 µg rh CA10 / CARPX enzyme at 100 ng/µl in 100 µL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/µg.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from 50 mM Tris, pH 7.5, 150 mM NaCl, mannitol or trehalose
Centrifuge the vial before opening. Reconstitute in sterile PBS, pH 7.4 to 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, store at -20°C.
CA10 Protein, CA-RPX Protein, Carbonic anhydrase X Protein, CARPX Protein, Cerebral protein 15 Protein, Cerebral protein-15 Protein, CARP X Protein, CA-RP X Protein, HUCEP-15 Protein
CA10 / Carbonic Andhydrase X is a protein that belongs to the carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. The protein encoded by this gene is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development. Multiple transcript variants encoding the same protein have been found for this gene.