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Human ANGPT1 / Angiopoietin-1 Recombinant (FLAG) Protein LS-G3732


Wt. Vol. Conc. Price
10 µg - - $295
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)

Most Popular ANGPT1 / Angiopoietin-1 Proteins

Human ANGPT1 / Angiopoietin-1 Recombinant (FLAG) Protein - LS-G3732
Hamster-Chinese CHO Cells
Unpurified / Lyophilized / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Functional Assay Image
Human ANGPT1 / Angiopoietin-1 Recombinant Protein - LS-G4911
Human HeLa Cells
Unpurified / Endotoxin Level: Less than 0.1 ng/µg of protein (less than 1EU/µg).
Human ANGPT1 / Angiopoietin-1 Recombinant Protein - LS-G16647
E. coli Expression System
Unpurified / Lyophilized

100% Guaranteed 100% Guaranteed
Recombinant Protein
ANGPT1 / Angiopoietin-1
~57kDa (SDS-PAGE)
Human angiopoietin-1 (aa 20-498) is fused at the N-terminus to a FLAG®-tag.
aa 20-498
CHO Cells
Greater than 90% by SDS-PAGE
Induces phosphorylation of Tie-2 in primary cultured human vascular endothelial cells (HUVECs). The protein forms mainly tetrameric structures.
Less than 0.1 EU/µg protein (determined by LAL method).
PBS, 0.02% CHAPS
Store at +4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid repeat freeze-thaw cycles.
For research use only.

About ANGPT1 / Angiopoietin-1

Q15389 NM_001146 NP_001137.2

ANGPT1 Protein, Angiopoietin 1 Protein, Angiopoietin-1 Protein, AGPT Protein, AGP1 Protein, ANG-1 Protein, ANG1 Protein, KIAA0003 Protein

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context.

Functional Assay

Functional Assay
Induction of Tie-2 phosphorylation in primary cultured HUVECs . Serum-starved human umbilical vein endothelial cells were treated with 200 ng/ml of BSA (Co) or 200 ng/ml of Angiopoietin-1 (human) (rec.) for 15 minutes. The phosphorylation of Tie-2 (Phospho-Tie-2) was measured after immunoprecipitation.

Functional Assay

Functional Assay
Glycerol spraying/low-angle rotary metal-shadowed specimens were imaged by transmission electron microscopy. Ang1 form mainly tetrameric structures, respectively.

Requested From: United States
Date Requested: 10/20/2016

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