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Catalog Number Size Price
LS-G118272-100 100 µg (1 mg/ml) $494 
HNE / Neutrophil Elastase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HNE / Neutrophil Elastase Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G118272

HNE / Neutrophil Elastase Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G118272

Available for shipment within the USA only
Description:
HNE / Neutrophil Elastase Protein LS-G118272 is an Over-Expression Lysate HNE / Neutrophil Elastase with Myc-DDK (Flag) tag(s). For Research Use Only
Price
Catalog Number
$494
LS-G118272-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
HNE / Neutrophil Elastase Protein LS-G118272 is an Over-Expression Lysate HNE / Neutrophil Elastase with Myc-DDK (Flag) tag(s). For Research Use Only

Specifications

Type
Over-Expression Lysate Protein
Target
HNE / Neutrophil Elastase
Synonyms
ELANE | Bone marrow serine protease | ELA2 | Elastase 2, neutrophil | Elastase-2 | HLE | Medullasin | Granulocyte-derived elastase | Neutrophil elastase | NE | Human leukocyte elastase | PMN elastase | PMN-E | SCN1 | Polymorphonuclear elastase | Elastase, neutrophil expressed | GE | Leukocyte elastase | Lysosomal elastase | HNE
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Myc-DDK (Flag)
Predicted Molecular Weight
28.52 kDa
AA Sequence
MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH(CloningSiteandTags:SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Unpurified
Usage Summary
Western blot: Spin down briefly and dilute with 2xSDS Sample Buffer provided (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT). Prior to SDS-PAGE fractionation, boil the lysate for 5-10 minutes.
Bio-Activity
Not Tested
Endotoxin
Not Tested
Presentation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Storage
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About HNE / Neutrophil Elastase
P08246 NM_001972 NP_001963.1

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Images

Western blot

HNE / Neutrophil Elastase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

HNE / Neutrophil Elastase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

HNE / Neutrophil Elastase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

HNE / Neutrophil Elastase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024