Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a small deletion at the C-terminus of the protein required for NLRC4 activation and is fused at the N-terminus to a His-tag.
Greater than 95% by SDS-PAGE
Activation of TLR5 in human epithelial cell assays based on NF-?B luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at a concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1µg/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages. It can be used as negative control for inflammasome signaling.
Less than 0.01 EU/µg protein (determined by LAL method).
Lyophilized from PBS.
Reconstitute with 100 µl sterile water.
Store lyophilized at -20°C for at least 6 months. Once reconstituted store at +4°C for immediate use.