Native Protein

fliC / Flagellin

Salmonella typhimurium

Unmodified

Salmonella typhimurium

Greater than 90% by SDS-PAGE

Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse.

Less than 0.1 EU/µg protein (determined by LAL method).

Lyophilized from PBS

Reconstitute with 100 µl sterile water.

Store lyophilized at -20°C for up to 6 months. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

NCBI: NP_416433.1