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fliC / Flagellin Protein (Native) - LS-G3883

Catalog
Size
Price
LS-G3883-10
10 µg
$275
Description
fliC / Flagellin Protein LS-G3883 is Native fliC / Flagellin in Salmonella typhimurium and is low in endotoxin Less than 0.01 EU/µg protein (determined by LAL method)..
Type
Native Protein
Target
fliC / Flagellin
Source Species
Salmonella typhimurium
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Not Tested
Endotoxin
Less than 0.01 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with 100 µl sterile water.
Storage
Store lyophilized at -20°C for up to 6 months. Once reconstituted store at 4°C for immediate use.
Restrictions
For research use only.
About fliC / Flagellin
NP_416433.1

Popular fliC / Flagellin Proteins

Protein
Source: E. coli Expression System
Tag: 6His,C-terminus
Format: Purified / Lyophilized
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Source: E. coli Expression System
Tag: 6His + SUMO,N-terminus
Format: Purified
Protein
Source: E. coli Expression System
Tag: His
Format: Purified / Lyophilized / Endotoxin Level: Less than 0.01 EU/µg protein (determined by LAL method).
Flagellin (AG-40B-0095) activates TLR5. Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
Source: Salmonella typhimurium Expression System
Tag: None
Format: Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).

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Functional Assay

Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).
Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).

Functional Assay

Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).
Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).

Functional Assay

Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).
Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).

Functional Assay

Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).
Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).

Requested From: United States
Date Requested: 3/26/2019
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