Recombinant Protein

IL-1B / IL-1 Beta

Human

Unmodified

The recombinant human IL1ß consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.

E. coli

E. coli

> 95 % as determined by SDS-PAGE.

Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 µg/mL (100µl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL. Measured by its binding ability in a functional ELISA. Immobilized human IL1B at 10 µg/mL (100 µl/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 µg/mL. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.

Not Tested

Lyophilized from sterile PBS, pH 7.4, 7.5% Trehalose

Reconstitute in 130ul of sterile water to a concentration of 0.15mg/ml

Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P01584 NCBI: NM_000576 NP_000567.1