Recombinant Protein

SARS-CoV-2 Spike Glycoprotein

Coronavirus SARS-CoV-2

Unmodified

51.5 kDa

HEK 293 Cells

Human

Greater than 95 % as determined by reducing SDS-PAGE.

A DNA sequence encoding the 2019-nCoV Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2µg/mL (100µL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag), the EC50 of Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50 ng/mL. Loaded Human ACE2 Protein (His tag) on SA Biosensor, can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) with an affinity constant of 0.562 nM as determined by Octet RED System.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from sterile PBS, pH 7.4

Long term: store at -20°C to -80°C for up to Lyophilized store for up to 12 months. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted store at less than -20°C for 3 months

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

NCBI: LR1719