Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
1. Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 µg/ml (100 µl/well) can bind mouse CD117 with a linear ranger of 1.28-32 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura,T. et al. 1989, J. Cell Physiol. 140:323.). The EC50 for this effect is typically 0.5-5 ng/ml. 2. Measured by its ability to inhibit Epodependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is 6-24 ng/mL.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from sterile 20 mM Tris, 50 mM NaCl, ph 7.4.
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1.