Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Purified / Lyophilized / Biologically Active / Endotoxin Level: Less than 0.06 EU/µg protein (determined by LAL method).
KITLG / SCF
Met 1-Ala 189
Greater than 95% by SDS-PAGE
1. Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 µg/ml (100 µl/well) can bind mouse CD117 with a linear ranger of 1.28-32 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura,T. et al. 1989, J. Cell Physiol. 140:323.). The EC50 for this effect is typically 0.5-5 ng/ml. 2. Measured by its ability to inhibit Epodependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is 6-24 ng/mL.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from sterile 20 mM Tris, 50 mM NaCl, ph 7.4.
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1.