Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Purified / Lyophilized / Biologically Active / Animal-Free / Endotoxin Level: Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Greater than 95% by SDS-PAGE
1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 10-50 ng/mL in the presence of Recombinant Rat GFR alpha-1/GDNF R alpha-1 His Chimera. 2. Immobilized rat His-GDNF (78-211) at 10 µg/ml (100 µl/well) can bind rat GFRA1-Fc, The EC50 of rat GFRA1-Fc is 20-46.6 ng/ml.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from 0.2 µm filtered 20 mM Tris, pH 7.4, 500 mM NaCl
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
GDNF Protein, HSCR3 Protein, HFB1-GDNF Protein, ATF Protein, HGDNF Protein
GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene.