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LSBio Functional ELISA Kits

Functional ELISA kits are a fast and cost-effective way to screen samples for the presence of functional proteins with specific activity characteristics. Each kit is ready-to-use with all the necessary reagents and a clear, concise protocol that will step you through the process, from sample preparation to analysis of the results.

Alphabetical Listing

Features:

Ready-to-use kit includes all necessary reagents
Direct quantitation of functional proteins
Excellent sensitivity, specificity, and reproducibility
Built on standard 96-well microtiter format
450 nm colorimetric detection

Kit Components:

Substrate Coated 96-well microliter plate
Sample Diluent
Positive Controls
Negative Controls
Wash Buffers
Primary Antibody
HRP-Conjugated Detection Antibody
TMB Substrate Solutions
Stop Buffer
Plate Sealer Sheets

Functional ELISA Kit Protocol

1) The kit comes with a 96-well microtiter plate that is pre-coated with the target antigen.

2) The sample is added to the well and the target protein binds to the protein substrate.

3) The wells are washed and a primary antibody is added which binds to the target protein.

4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody.

5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. The plate can then be read in a spectrophotometer at a wavelength of 450 nm.

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