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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
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LSBio Functional ELISA Kits

ELISA Kit Image
Functional ELISA kits are a fast and cost-effective way to screen samples for the presence of functional proteins with specific activity characteristics. Each kit is ready-to-use with all the necessary reagents and a clear, concise protocol that will step you through the process, from sample preparation to analysis of the results.




Features:

  • Ready-to-use kit includes all necessary reagents
  • Direct quantitation of functional proteins
  • Excellent sensitivity, specificity, and reproducibility
  • Built on standard 96-well microtiter format
  • 450 nm colorimetric detection

Kit Components:

  • Substrate Coated 96-well microliter plate
  • Sample Diluent
  • Positive Controls
  • Negative Controls
  • Wash Buffers
  • Primary Antibody
  • HRP-Conjugated Detection Antibody
  • TMB Substrate Solutions
  • Stop Buffer
  • Plate Sealer Sheets

Functional ELISA Kit Protocol

Functional ELISA Overview
1) The kit comes with a 96-well microtiter plate that is pre-coated with the target antigen. 2) The sample is added to the well and the target protein binds to the protein substrate. 3) The wells are washed and a primary antibody is added which binds to the target protein. 4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. 5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. The plate can then be read in a spectrophotometer at a wavelength of 450 nm.