Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy
- Details about how to buy our products.
Orders@LSBio.com
- To submit a new order.
Customer.Support@LSBio.com
- To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com
- To request technical information about an LSBio product or its applications
Sales@LSBio.com
- To request information about distribution agreements, or general business development.
Worldwide Distributors List
- To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
LSBio Functional ELISA Kits
Functional ELISA kits are a fast and cost-effective way to screen samples for the presence of functional proteins with specific activity characteristics.
Each kit is ready-to-use with all the necessary reagents and a clear, concise protocol that will step you through the process, from sample preparation to analysis of the results.
Features:
- Ready-to-use kit includes all necessary reagents
- Direct quantitation of functional proteins
- Excellent sensitivity, specificity, and reproducibility
- Built on standard 96-well microtiter format
- 450 nm colorimetric detection
Kit Components:
- Substrate Coated 96-well microliter plate
- Sample Diluent
- Positive Controls
- Negative Controls
- Wash Buffers
- Primary Antibody
- HRP-Conjugated Detection Antibody
- TMB Substrate Solutions
- Stop Buffer
- Plate Sealer Sheets
Functional ELISA Kit Protocol
| 1) The kit comes with a 96-well microtiter plate that is pre-coated with the target antigen. | 2) The sample is added to the well and the target protein binds to the protein substrate. | 3) The wells are washed and a primary antibody is added which binds to the target protein. | 4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. | 5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. The plate can then be read in a spectrophotometer at a wavelength of 450 nm. |
