Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 610/620
20700 44th Avenue W
Lynnwood, WA 98036
1) The kit comes with a 96-well microtiter plate that is pre-coated with the target antigen. | 2) The sample is added to the well and the target protein binds to the protein substrate. | 3) The wells are washed and a primary antibody is added which binds to the target protein. | 4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. | 5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. The plate can then be read in a spectrophotometer at a wavelength of 450 nm. |