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LSBio Functional ELISA Kits

ELISA Kit Image
Functional ELISA kits are a fast and cost-effective way to screen samples for the presence of functional proteins with specific activity characteristics. Each kit is ready-to-use with all the necessary reagents and a clear, concise protocol that will step you through the process, from sample preparation to analysis of the results.




Features:

  • Ready-to-use kit includes all necessary reagents
  • Direct quantitation of functional proteins
  • Excellent sensitivity, specificity, and reproducibility
  • Built on standard 96-well microtiter format
  • 450 nm colorimetric detection

Kit Components:

  • Substrate Coated 96-well microliter plate
  • Sample Diluent
  • Positive Controls
  • Negative Controls
  • Wash Buffers
  • Primary Antibody
  • HRP-Conjugated Detection Antibody
  • TMB Substrate Solutions
  • Stop Buffer
  • Plate Sealer Sheets

Functional ELISA Kit Protocol

Functional ELISA Overview
1) The kit comes with a 96-well microtiter plate that is pre-coated with the target antigen. 2) The sample is added to the well and the target protein binds to the protein substrate. 3) The wells are washed and a primary antibody is added which binds to the target protein. 4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. 5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. The plate can then be read in a spectrophotometer at a wavelength of 450 nm.



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