Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B5752-100 100 µg (0.5 mg/ml) $485 
WT1 / Wilms Tumor 1 Antibody - Anti-WT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

PathPlus™ Monoclonal WT1 / Wilms Tumor 1 Antibody (clone 1E9, IHC, WB) LS‑B5752

PathPlus™ Monoclonal WT1 / Wilms Tumor 1 Antibody (clone 1E9, IHC, WB) LS‑B5752

Note: This antibody replaces LS-C133975
Antibody:
Mouse Monoclonal to Human WT1 / Wilms Tumor 1
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B5752-100
100 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human WT1 / Wilms Tumor 1
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
WT1 is a transcription factor essential to normal development of the human urogenital system. It is expressed in the fetal kidney and mesothelium, as well as in certain malignancies. It can be used to identify ovarian carcinoma and mesothelioma; levels of WT1 expression can also indicate disease progression in acute myeloid lymphoma. Furthermore, it is a potential target for cancer vaccines. In normal tissue, WT1 is positive in fallopian tube epithelium, smooth muscle cells and endothelial cells (nuclear and weak cytoplasmic localization), and testis. In cancer, it is positive in Wilms' tumor, malignant mesotheliomas, and serous ovarian adenocarcinomas. References: Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13824-9, PMID: 20631300; Cell. 1993 Aug 27;74(4):679-91, PMID: 8395349; Sexual Development. 2007;1(4):238-54, PMID: 18391535; Oncologist. 2012;17(2):250-9, PMID: 22291091; BMC Cancer. 2006 Apr 11;6:90, PMID: 16606472
Target
Human WT1 / Wilms Tumor 1
Synonyms
WT1 | GUD | EWS-WT1 | Amino-terminal domain of EWS | WAGR | WIT-2 | Wilms tumor protein | AWT1 | Wilms tumor 1 | WT33 | NPHS4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
1E9
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
WT1 (NP_000369, 349 aa ~ 439 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human WT1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WT1 / Wilms Tumor 1
P19544 NM_024426 NP_077744.3

Validation

WT1 / Wilms Tumor 1 Antibody - Anti-WT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-WT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-WT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 2/22/2020