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PathPlus™ Tyrosinase Antibody Monoclonal ELISA,ICC,IF,IHC,WB LS‑B2473

Catalog Number Size Price
LS-B2473-250 250 µl (0.2 mg/ml) $485 
Anti-Tyrosinase antibody IHC of human skin, melanocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

PathPlus™ Tyrosinase Antibody Monoclonal ELISA,ICC,IF,IHC,WB LS‑B2473

Note: This antibody replaces LS-C18403
Antibody:
Mouse Monoclonal to Human Tyrosinase
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Cat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B2473-250
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Tyrosinase
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Cat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
Tyrosinase (TYR) is an enzyme that catalyzes steps in the conversion of tyrosine to melanin. Mutations in TYR results in oculocutaneous albinism, and polymorphisms results in variations in skin pigmentation. The protein is found in melanocytes in the skin and retina, and is useful to identify melanocytic neoplasms such as melanomas. It is also found in subsets of renal angiomyolipomas and PEComas. PNAS USA 1995 92:8125; Arch Path Lab Med 2007 131(1):122; Arch Path Lab Med 2009 133:648
Target
Human Tyrosinase
Host
Mouse
Reactivity
Human, Cat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
T311
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant tyrosinase protein. Epitope: 1-433.
Specificity
Recognizes a cluster of proteins between 70-80kD, identified as human tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: canine and feline. Weak cross-reactivity with mouse.
Usage
Immunohistochemistry: LS-B2473 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2473 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Tyrosinase
P14679 NM_000372 NP_000363.1

Validation

Anti-Tyrosinase antibody IHC of human skin, melanocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ Validated
Anti-Tyrosinase antibody IHC of human skin, melanocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Tyrosinase antibody IHC of human skin, melanocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Requested From: United States
Date Requested: 9/18/2019