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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5782-100 | 100 µl (0.5 mg/ml) | $485 |
1 of 2
2 of 2
PathPlus™ Polyclonal Rabbit anti‑Human PDPN / Podoplanin Antibody (C‑Terminus, IHC, WB) LS‑B5782
PathPlus™ Polyclonal Rabbit anti‑Human PDPN / Podoplanin Antibody (C‑Terminus, IHC, WB) LS‑B5782
Note: This antibody replaces LS-C102112
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
PDPN (podoplanin) is mucin-type membrane glycoprotein with expression in a diverse range of tissues and cell types including lung alveolar cells, kidney podocytes, lymphatic endothelial cells, and neural tissues. PDPN is a required protein in the development of the lungs, lymphatic system and the heart, and it separately may play a role in immune response to fungal infections. In immunohistochemistry, PDPN has cytoplasmic and membranous staining in the lymphatic endothelium, breast myoepithelium, podocytes, dendritic cells and mesothelium. It is a sensitive and specific marker for the lymphatic endothelium, and it is used as an endothelial cell marker in determining lymphatic invasion in tumors.
References: Clin Inv. 118 (11): 3725–37, PMID: 18924607; Frontiers in Immunology. 2012. 3: 283. doi:10.3389/fimmu.2012.00283, PMID: 22988448
Target
Human PDPN / Podoplanin
Synonyms
PDPN | AGGRUS | HT1alpha-1 | Glycoprotein 36 | Glycoprotein, 36-KD | gp36 | gp38 | OTS8 | PA2.26 | PA2.26 antigen | Podoplanin | T1A | gp40 | HT1A-1 | HT1alpha-2 | p38 | T1-alpha | T1A-2
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from C-Terminus of human PDPN (Q86YL7-3, NP_006465). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (92%); Marmoset (84%).
Epitope
C-Terminus
Specificity
Human PDPN / Podoplanin
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PDPN / Podoplanin
Validation
Anti-Podoplanin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Podoplanin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
PathPlus™ PDPN / Podoplanin Antibody (C-Terminus) for IHC, WB/Western LS-B5782 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, IHC - Frozen
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Human
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IHC, IHC - Paraffin, Western blot, ELISA
Species:
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IHC, IHC - Paraffin, IHC - Frozen
Species:
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Immunofluorescence, Western blot, Immunoprecipitation
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Human
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
