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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PathPlus™ PDPN / Podoplanin Antibody C‑Terminus Polyclonal IHC,WB LS‑B5782

Catalog Number Size Price
LS-B5782-100 100 µl $331 
Anti-Podoplanin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PDPN / Podoplanin antibody Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Podoplanin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PDPN / Podoplanin antibody Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PathPlus™ PDPN / Podoplanin Antibody C‑Terminus Polyclonal IHC,WB LS‑B5782

Note: This antibody replaces LS-C102112
Antibody:
Rabbit Polyclonal to Human PDPN / Podoplanin
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$331 
LS-B5782-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PDPN / Podoplanin
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
PDPN (podoplanin) is mucin-type membrane glycoprotein with expression in a diverse range of tissues and cell types including lung alveolar cells, kidney podocytes, lymphatic endothelial cells, and neural tissues. PDPN is a required protein in the development of the lungs, lymphatic system and the heart, and it separately may play a role in immune response to fungal infections. In immunohistochemistry, PDPN has cytoplasmic and membranous staining in the lymphatic endothelium, breast myoepithelium, podocytes, dendritic cells and mesothelium. It is a sensitive and specific marker for the lymphatic endothelium, and it is used as an endothelial cell marker in determining lymphatic invasion in tumors. Clin Inv. 118 (11): 3725–37, PMID: 18924607; Frontiers in Immunology. 2012. 3: 283. doi:10.3389/fimmu.2012.00283, PMID: 22988448
Target
Human PDPN / Podoplanin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from C-Terminus of human PDPN (Q86YL7-3, NP_006465). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (92%); Marmoset (84%).
Blocking Peptide
LS-E16362 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5782. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human PDPN / Podoplanin
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDPN / Podoplanin
Q86YL7 NM_006474 NP_006465.3

Validation

Anti-Podoplanin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PathPlus™ Validated
Anti-Podoplanin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Podoplanin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 8/17/2019
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