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Catalog Number Size Price
LS-B7135-50 50 µl $460 
Vimentin Antibody - Anti-Vimentin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
Vimentin Antibody - Immunoblot Analysis of cell Lysates
Vimentin Antibody - Anti-Vimentin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
Vimentin Antibody - Immunoblot Analysis of cell Lysates
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2 of 2

PathPlus™ Monoclonal Mouse anti‑Human Vimentin Antibody (clone 35E2, IHC, WB) LS‑B7135

PathPlus™ Monoclonal Mouse anti‑Human Vimentin Antibody (clone 35E2, IHC, WB) LS‑B7135

Note: This antibody replaces LS-C62127
Antibody:
Vimentin Mouse anti-Human Monoclonal (35E2) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B7135-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Vimentin Mouse anti-Human Monoclonal (35E2) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Vimentin (VIM) is an intermediate filament component of the cytoskeleton of mesenchymal cells. It is important for maintaining cell integrity and shape. Vimentin also regulates cell migration via cytoskeletal rearrangements and is involved in regulating autophagy. In immunohistochemistry, it is positive in the cytoplasm of cells of mesenchymal origin, such as lymphocytes, endothelial cells, astrocytes, epidermal Langerhans cells, macrophages, and interstitial stromal cells. In cancer, it is positive in lymphomas and stromal tumors and is helpful for identifying melanomas, schwannomas and tumors of mesenchymal origin. References: Am J Surg Pathol. 2011;35:1722, PMID: 21997693; F1000Res. 2018 Nov 15;7. pii: F1000 Faculty Rev-1796, PMID: 30505430; PLoS One. 2019 Jan 30;14(1):e0209665, PMID: 30699149; Toxicol Pathol. 2010 Jan;38(1):131-41, PMID: 20028992
Target
Human Vimentin
Synonyms
VIM | Vimentin | vimenin
Host
Mouse
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
35E2
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human protein purified from E. coli (His-Vimentin).
Applications
  • IHC
  • IHC - Paraffin (1:400)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
HEPES, 0.15 M NaCl, 0.03% Sodium Azide, 50% Glycerol, 0.01% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Vimentin
P08670 NM_003380 NP_003371.2

Validation

Vimentin Antibody - Anti-Vimentin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
Anti-Vimentin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
Anti-Vimentin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.

LSBio Ratings

PathPlus™ Vimentin Antibody (clone 35E2) for IHC, WB/Western LS-B7135 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/25/2022