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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2471-50 | 50 µg (1 mg/ml) | $485 |
PathPlus™ Monoclonal Mouse anti‑Human SERPINF1 / PEDF Antibody (aa196‑347, IHC, WB) LS‑B2471
PathPlus™ Monoclonal Mouse anti‑Human SERPINF1 / PEDF Antibody (aa196‑347, IHC, WB) LS‑B2471
Note: This antibody replaces LS-C17230
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
SERPINF1 (PEDF) is a neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. It is a potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human SERPINF1 / PEDF
Synonyms
SERPINF1 | EPC-1 | OI12 | PEDF | Serpin F1 | PIG35 | OI6
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human GST-PEDF fusion protein. The sequence corresponding to aa196-347 of human PEDF tagged with GST on the N-Terminus was expressed in E. coli. GST-PEDF is about 46kD on SDS-PAGE.
Epitope
aa196-347
Specificity
Recognizes human GST-PEDF fusion protein with a MW of ~46kD.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- IHC - Frozen
- ICC
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2471 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2471 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: store at 4°C for up to 2 weeks. Long term: aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SERPINF1 / PEDF
Validation
Anti-PEDF antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PEDF antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ SERPINF1 / PEDF Antibody (aa196-347) for IHC, ICC, WB/Western, ELISA LS-B2471 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Differentiation of human pluripotent stem cells to retinal pigmented epithelium in defined conditions using purified extracellular matrix proteins. Rowland TJ, Blaschke AJ, Buchholz DE, Hikita ST, Johnson LV, Clegg DO. Journal of tissue engineering and regenerative medicine. 2013 August;7:642-653.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
