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Catalog Number Size Price
LS-A9486-50 50 µg (1 mg/ml) $440 
PLA2G3 Antibody - Brain, Glioblastoma
PLA2G3 Antibody - Brain, Hypothalamus, neurons
PLA2G3 Antibody - Anti-PLA2G3 antibody IHC of human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
PLA2G3 Antibody - Brain, Glioblastoma
PLA2G3 Antibody - Brain, Hypothalamus, neurons
PLA2G3 Antibody - Anti-PLA2G3 antibody IHC of human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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PathPlus™ Polyclonal Rabbit anti‑Human PLA2G3 Antibody (Internal, IHC) LS‑A9486

PathPlus™ Polyclonal Rabbit anti‑Human PLA2G3 Antibody (Internal, IHC) LS‑A9486

Note: This antibody replaces LS-C90219
Antibody:
PLA2G3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Bovine, Pig
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$440
LS-A9486-50

Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PLA2G3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Bovine, Pig
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Phospholipase A2, group III (PLA2G3) belongs to a superfamily of intracellular and secreted enzymes that catalyze the hydrolysis of glycerophospholipids at the sn-2 position to release fatty acids and lysophospholipids. PLA2G3 enzymes were originally identified in invertebrates such as bees and scorpions. PLA2G3 has been shown to be a calcium dependent enzyme. It consists of a central group III sPLA2 domain flanked by unique N- and C-terminal domains that are proteolytically removed in most tissues. The sPLA2 domain is sufficient for catalytic activity. PLA2G3 is preferentially expressed in the microvascular endothelium of tissues with inflammation, ischemic injury and cancer. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human PLA2G3
Synonyms
PLA2G3 | GIII-SPLA2 | Phospholipase A2, group III | SPLA2-III | GIII sPLA2 | SPLA2III
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Pig (tested or 100% immunogen sequence identity)
Predicted
Mouse, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 12 amino acid peptide from internal region of human PLA2G3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Elephant, Panda, Pig (100%); Mouse, Horse, Rabbit (92%); Bat, Dog, Opossum (83%).
Epitope
Internal
Specificity
Human PLA2G3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PLA2G3
Q9NZ20 NM_015715 NP_056530.2

Validation

PLA2G3 Antibody - Brain, Glioblastoma
Brain, Glioblastoma
Brain, Glioblastoma

PLA2G3 Antibody - Brain, Hypothalamus, neurons
Brain, Hypothalamus, neurons
Brain, Hypothalamus, neurons

PLA2G3 Antibody - Anti-PLA2G3 antibody IHC of human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PLA2G3 antibody IHC of human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PLA2G3 antibody IHC of human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ PLA2G3 Antibody (Internal) for IHC LS-A9486 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Phospholipase A2 expression in coronary thrombus is increased in patients with recurrent cardiac events after acute myocardial infarction. Takahashi S, Suzuki K, Watanabe Y, Watanabe K, Fujioka D, Nakamura T, Obata JE, Kawabata K, Mishina H, Kugiyama K. International journal of cardiology. 2013 Oct;168:4214-21.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/10/2022